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Good’s buffers as a basis for developing self-buffering and biocompatible ionic liquids
Every day scientists in laboratories across the world sit at their desks and painstakingly design experiments in the hope of making a discovery that will change how we think about a biological process. Because biological processes such as enzymatic activity are dependent on pH, one critical aspect of the experimental design is choosing a buffering system that will help maintain a stable pH without altering the results.
And, often, it is the choice of buffer that makes or breaks the experiment. It is possible that the buffer you are using in your lab might be the reason your experiment is failing. Here, you will find how a buffering system works, a description of the characteristics of a good buffer and a list of possible applications and characteristics of the most commonly used biological buffer series.
A buffer consists of a weak acid (proton donor, HA) and its conjugate base (proton acceptor, A -). In water, HA can dissociate into A- and H+. H+ then reacts with water to form H3O+. In the aqueous buffer solution, H3O+, HA and H+ exist in equilibrium with each other. The buffering mechanism consists of two reversible reactions where the concentration of proton donor and proton acceptor are equal.
Then, when a strong acid or base is introduced into this system by the scientist or by enzymatic activity during the experiment, the new ions from the introduced acid or base (H + or OH-) are absorbed by the buffer and the pH remains stable preventing changes in protein structure and function.
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